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Image Search Results
Journal: Endocrinology
Article Title: Subdomain 2, Not the Transmembrane Domain, Determines the Dimerization Partner of Growth Hormone Receptor and Prolactin Receptor
doi: 10.1210/en.2017-00469
Figure Lengend Snippet: Replacement of GHR S2 or TMD with S2 or TMD of PRLR does not impair GH signaling. (a) Schematic diagram of domain-swapped human GHR/PRLR chimeras tethered with a Nluc/Cluc fragment at their cytoplasmic tails. GHR is in gray. PRLR is in orange. GHR(PRLRS2-TMD) is GHR with the S2 and TMD replaced with those of PRLR. GHR(PRLRS2) is GHR with its S2 replaced with the S2 of PRLR. GHR(PRLRTMD) is GHR with its TMD replaced with the TMD of PRLR. (b) GHR(PRLRS2-TMD), GHR(PRLRS2-TMD)-Nluc, and GHR(PRLRS2-TMD)-Cluc allow acute GH signaling. γ2A-JAK2 cells were transiently transfected with 3 μg GHR, 4.5 μg PRLR, 3 μg GHR(PRLRS2-TMD), 4.5 μg GHR(PRLRS2-TMD)-Nluc, 4 μg GHR(PRLRS2-TMD)-Cluc, or 3 μg empty vector per 6-cm dish, respectively, treated with ±GH, and analyzed by immunoblotting with anti-GHR (anti-GHRcyt-AL47, polyclonal antibody against the rabbit GHR intracellular domain), anti-PRLR (anti-PRLRcyt-AL84, polyclonal antibody against the human PRLR intracellular domain), anti-pJAK2, and anti-JAK2 (anti-JAK2AL33). (c) GHR(PRLRS2), GHR(PRLRS2)-Nluc, and GHR(PRLRS2)-Cluc allow acute GH signaling. γ2A-JAK2 cells were transiently transfected with 3 μg GHR, 4.5 μg PRLR, 3 μg GHR(PRLRS2), 4.5 μg GHR(PRLRS2)-Nluc, 4 μg GHR(PRLRS2)-Cluc, or 3 μg empty vector per 6-cm dish, respectively, treated with ±GH, and analyzed by immunoblotting with anti-GHR, anti-PRLR, anti-pJAK2, and anti-JAK2. (d) GHR(PRLRTMD), GHR(PRLRTMD)-Nluc, and GHR(PRLRTMD)-Cluc allow acute GH signaling. γ2A-JAK2 cells were transiently transfected with 3 μg GHR, 4.5 μg PRLR, 3 μg GHR(PRLRTMD), 4.5 μg GHR(PRLRTMD)-Nluc, 3 μg GHR(PRLRTMD)-Cluc, or 3 μg empty vector per 6-cm dish, respectively, split into 6-well plates, treated with ±GH, and analyzed by immunoblotting with anti-GHR, anti-PRLR, anti-pJAK2, and anti-JAK2.
Article Snippet:
Techniques: Transfection, Plasmid Preparation, Western Blot
Journal: International Journal of Molecular Medicine
Article Title: Protective effect of ginseng extract and total ginsenosides on hematopoietic stem cell damage by inhibiting cell apoptosis and regulating the intestinal microflora
doi: 10.3892/ijmm.2024.5455
Figure Lengend Snippet: Protein expression of Klf4 and Pbx1 as determined by immunohistochemical staining in the mouse bone marrow (magnification, ×400). (A) KLF4 and (B) pbx1 staining. The arrows show locations of positive Klf4/Pbx1 expression. GE, ginseng extract; TG, total ginsenosides; TP, total polysaccharides; KLF4, Krüppel-like factor 4.
Article Snippet: Rabbit anti-mouse Klf4 (cat. no. 11880-1-AP; Proteintech Group, Inc.) and
Techniques: Expressing, Immunohistochemical staining, Staining